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Biological Activity
Valor FGF2 146 is functionally validated using ERK phosphorylation and cell proliferation assays in NIH-3T3 cells, an FGF-responsive murine fibroblast line commonly used as the reference standard for FGF2 bioactivity testing. Biological activity is confirmed by dose-dependent induction of ERK1/2 phosphorylation and cell proliferation in a mitogenic-dependent manner.

Each lot is tested independently to ensure consistent bioactivity and lot-to-lot reproducibility.

FGF2 Stability Considerations in Cell Culture
Recombinant FGF2 has a half-life of approximately 10 hours at 37°C in cell culture media. To maintain sustained FGFR signaling during stem cell maintenance, supplemented cell culture media should be replaced every 24-48 hours. 

To preserve Valor FGF2 146 bioactivity:

• Avoid repeated freeze-thaw cycles by aliquotting into single-use volumes immediately after the first thaw to maintain bioactivity and protein integrity.

• Store at -80°C; do not store at -20°C, to avoid temperature fluctuations that lead to protein degradation.

• Avoid exposing reagent to room temperature exposure during reagent preparation.

• Low-binding tubes and pipette tips should be used to avoid product loss.

• Adding 0.1% bovine serum albumin (BSA) as a carrier protein prevents surface adsorption. 

• Do not vortex; gently flick or triturate with a pipette tip. 

Recommended Working Concentrations
Working concentrations of FGF2 vary depending on cell type, culture conditions, and experimental objectives: